C2 Plus Confocal Microscope System

by Nikon

Built on a reputation of incredible stability coupled with superior optical technologies, the C2+ with its host of functions and various analytical capabilities is the perfect tool for a new microscope, or as a new accessory to a Nikon imaging system. Now fully controlled by NIS-Elements imaging software, the system includes four channel confocal fluorescence imaging, and vastly expanded spectral capabilities with the ability to capture and unmix data acquired at any channel resolution across the entire detector bandwidth.

System Highlights

    • Amazing image quality, flexibility, and modularity
    • Optimized scanning accuracy and speed
    • Imaging speeds up to 100fps
    • Four channel confocal fluorescence
    • True "One Shot" quantitative 32 channel spectral imaging with accuracy and sensitivity
    • 2.5nm, 5nm, or 10nm spectral resolution
    • Linear spectral unmixing of 3 channel or 4 channel images from the standard fluorescence detector

      High Efficiency Scanning Heads and Detectors

      The C2+ fits all Nikon microscopes with the smallest scan head footprint on the market. The system employs high precision mirrors and optically ideal circular pinholes, enabling noiseless, high contrast and high quality confocal imaging. The spectral detector of the C2
      enables high speed imaging using simultaneous quantitative 32-channel acquisition. Signal loss has been minimized while imaging of fluorescence spectra in real colors is possible by host of innovations for accurately correcting spectral data.

      CFI Apochromat S Series

      These high NA objectives are ideal for confocal imaging with correction of chromatic aberrations over a wide wavelength range, from ultra violet to infrared. Transmission is increased through the use of Nikon's exclusive Nano Crystal Coat technology.

      CFI Apochromat TIRF Series

      These objectives boast an unprecedented NA of 1.49 (using a standard coverslip and immersion oil), the highest resolution among Nikon objectives. The temperature correction ring corrects image quality affected by temperature change in the range of 23&degC to 37&degC

      NIS-Elements Imaging Software

      The NIS-Elements platform facilitates the entire workflow from live analysis and full control of all experimental parameters during acquisition, maximum and minimum intensity projections over time and Z-axis, Z depth display and volume rendering, image analysis, processing and data management.

      NIS-Elements' core functionalities include several customizable controls for device control and presets, enhanced multi-camera support and control, intensity correction through Z, combining Extended Depth of Focus with High Dynamic Range, merging Large Image Stitching with Z Capture, and definition of stage travel based on analysis results . In addition, new options are available for 2D and 3D Deconvolution, new tracking tools linked with regions of interest and numerous new options for data export.

      Multimode Imaging System TIRF Photoactivation

      A TIRF laser illumination module and a photoactivation module are integrated to enable both imaging of single molecules with extremely high S/N ratio and imaging of the fluorescence characteristic changes of photoactivated and photo-convertible fluorescent protein.

      High Definition Diascopic DIC Imaging

      The C2
      handles simultaneous 3-channel fluorescence or simultaneous 3-channel and diascopic DIC observation. High quality DIC images and fluorescence images can be superimposed to aid in image analysis such as locating fluorescence labels.

      Macro Confocal System

      With high-definition, wide field of view images larger than 1cm at exceptional high S/N ratios, the AZ-C2
      allows for imaging of whole-mount specimens, such as embryos, in a single shot. It offers a combination of low and high magnification objective lenses, optical zoom and a confocal scanning zoom function, enabling continuous imaging from macro to micro. Moreover, the AZ-C2
    allows deep imaging of in-vivo whole specimens.

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