A1MP Multiphoton Confocal Microscope

by Nikon

Nikon's A1 MP is a unique multiphoton imaging system featuring a high resolution galvanometer scanner. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.

Deep Imaging with High-sensitivity Non-Descanned Detector

Nikon's newly developed 4-channel NDD for multiphoton microscopy allows imaging deep into a specimen. A detector* with very high sensitivity and a wider sensitive area than conventional models is placed close to the back aperture of the objective where image formation of the specimen takes place. This configuration improves the detection efficiency for scattered fluorescence, and enables the capture of signals from deep within a living organism more clearly and more reliably with higher S/N and less aberrations.

* Detecting as much scattered fluorescence as possible is important for observation of deep parts in a specimen. The detection depth varies depending on the detector sensitivity, size and installation position.

High-precision Unmixing

Spectral detection and unmixing by the 4-channel NDD for multiphoton microscopy allows clear and high-contrast images of areas deep within a thick specimen can be captured.

Multiphoton Laser Beam Alignment With a Single Click

When the multiphoton laser wavelength or group velocity dispersion precompensation is changed, the multiphoton laser beam positional pointing may drift, resulting in uneven intensity of images, or a slight misalignment of fluorescence images that are produced by a visible light laser and a multiphoton laser.

Since the multiphoton laser is invisible to the eye, the laser beam alignment has been difficult and potentially dangerous for users, especially at wavelengths longer than 800 nm. Nikon A1R MP's newly developed auto laser alignment function automatically corrects multiphoton laser misalignments with a single click.

Sharper, Brighter Imaging with New High NA Objectives

The new water immersion objectives offer superior chromatic aberration correction throughout a wide wavelength range and high transmission covering from near-ultraviolet to near-infrared range, made possible by Nikon's proprietary Nano Crystal Coat*. CFI Plan Apo IR 60x WI with an NA of 1.27 - the world's highest for a 60x water immersion objective - offers high-contrast imaging with excellent resolution and brightness.

* An ultra-low refractive index film that was initially developed for Nikon's IC steppers. With its coarse structure - nano particles are arranged in a spongy composition - thus the Nano Crystal Coat boosts the transmission of light over a wide spectral wavelength range.

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